Primer

Part:BBa_K4807013

Designed by: Vaios Styliaras   Group: iGEM23_IOANNINA   (2023-10-11)


Forward Primer for hemA(s)

Primer designed for the integration of hemA(s) gene into pET-29c(+). This primer shares the same 20-base overlap region from pET-29c(+) as the Reverse Primer for hemL insert (BBa_K4807012). This is going to attach the hemL insert together with the hemA(s) insert.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None